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3D-Printed Chitosan-Based Scaffolds with Scutellariae baicalensis Extract for Dental Applications.The plant material Scutellariae baicalensis radix, which is rich in flavones (baicalin), possesses antibacterial, antifungal, antioxidant, and anti-inflammatory properties. Health Benefits taked to develop a 3D-printed chitosan-established hydrogel rich in Scutellariae baicalensis extract as an innovative approach for the personalized treatment of periodontal diseases. Chitosan-based hydrogels were groomed, and the printability of the prepared hydrogels was determined. The hydrogel with 2% w/v of high molecular-weight chitosan (CS), 2% w/v gelatin (Gel), and 10% w/w of extract (Ex) gived the best printability, creating smooth and uniform scaffolds. It was proved that the CS/Gel/Ex hydrogel was stabilized by hydrogen bails and persisted in amorphous dispersion in the 3D-printed structures (substantiated by ATR-FTIR and XRPD). Due to the amorphization of the active substance, a significant increase in the release of baicalin in vitro was remarked. It was manifested that there was an initial burst release and a continuous release profile (n = 3). Higuchi kinetic was the most likely baicalin release kinetic. The second fit, the Korsmeyer-Peppas kinetics model, pictured twined diffusion of the active ingredient in the hydrated matrix and polymer relaxation molded release, with n values roving from 0 to 0. The anti-inflammatory properties of 3D-published scaffolds were measured as the ability to inhibit the activity of the hyaluronidase enzyme. Activity was valued as IC(50) = 63 ± 4 mg hydrogel/mL (n = 6). Cytotoxicity tests demonstrated the biocompatibility of the material. After 24 h of exposure to the 2CS/2Gel/10Ex scaffold, fibroblasts transmigrated toward the scratch, concluded the “wound” by 97%, and significantly accelerated the wound healing process. The consequences render the 3D-printed CS/Gel/extract scaffolds as potential nominees for treating periodontal diseases.Analysis of molecular structure and topological attributes of chitosan sequestered from crab shell and mushroom.This investigation aimed to scrutinize the chemical and structural analogies between chitosan evoked from crab exoskeleton (High Molecular Weight Chitosan, HMWC) and chitosan obtained from mushrooms (Mushroom-deducted Chitosan, MRC), and to assess their biological functionalities. Selenium leading hydrolysates from the hydrolysis of HMWC by chitosanase were categorised as chitosan oligosaccharides (csCOS), while those from MRC were denoted as mrCOS. The molecular weights (MW) of csCOS and mrCOS were ascertained utilising Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) mass spectrometry structural resemblances of csCOS and mrCOS were taxed utilizing X-ray powder diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy no apparent structural disparity between csCOS and mrCOS was noted in terms of the glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) composition ratios. Consequently, the enzymatic activities of chitosanase for HMWC and MRC exhibited remarkable similarity. A topological examination was executed between the enzyme and the substrate to deduce the alteration in MW of COSs watching enzymatic hydrolysis the evaluation of antioxidant activity for each COS revealed insignificance in the structural disparity between HMWC and MRC. In summary, grounded on the chemical structural similarity of HMWC and MRC, we propose the potential substitution of HMWC with MRC, incorporating diverse biological functionalities.Biodegradability Study of Modified Chitosan Films with Cinnamic Acid and Ellagic Acid in Soil.Currently, natural polymer materials with bactericidal props are extremely popular although the biopolymer material itself is biodegradable, its enrichment with bactericidal compounds may affect the efficiency of biodegradation by natural soil microflora the primary objective of this study was to evaluate the utility of fungi going to the genus Trichoderma in facilitating the degradation of chitosan film changed with cinnamic acid and ellagic acid in the soil environment.